Journal: PLoS ONE

Article Title: Stromal Cells Positively and Negatively Modulate the Growth of Cancer Cells: Stimulation via the PGE2-TNFα-IL-6 Pathway and Inhibition via Secreted GAPDH-E-Cadherin Interaction

doi: 10.1371/journal.pone.0119415

Figure Lengend Snippet: ( A ) MKN-7 and MKN-74 cells were cultured alone (Mono) or co-cultured with Hs738 (Cocul) for 3 days in the presence of MEK inhibitor I or U0126. Hs738 cells were cultured with the inhibitors for 2 days and CM was prepared. Both gastric cancer lines were cultured in the CM for 3 days. Cell growth was determined by measuring GFP fluorescence intensity. The values are means ± s.d. (n = 3). Cell growth is expressed as a percentage of the value without test compounds in each culture condition. ( B ) Hs738 cell extracts were incubated with b-MEK inh-pretreated streptavidin resin in the presence or absence of MEK inhibitor I (M) or U0126 (U) and the bound proteins were analyzed by Western blotting. L, 1/50 of loaded cell extracts. ( C ) Hs738 cells were cultured with MEK inhibitor I for 2 days and the cell lysates were analyzed by Western blotting. ( D ) Hs738 cells were cultured with MEK inhibitor I for 2 days and the concentrations of IL-6 and CXCL1 in the CM were determined. The values are means ± s.d. (n = 3). ( E ) Hs738 cells were treated with siRNA specific for RPL-18A (siRPL) or negative control (siCont) for 2 days and then re-inoculated followed by further culture for 2 days. The cell lysates were analyzed by Western blotting and the amounts of IL-6 in the CM were determined. The values are means ± s.d. (n = 3).

Article Snippet: Anti-human IL-6 neutralizing antibody (MAB206), recombinant human IL-6 (206-IL), and recombinant human CXCL1 (275-CR/CF) were purchased from R&D Systems.

Techniques: Cell Culture, Fluorescence, Incubation, Western Blot, Negative Control

Journal: PLoS ONE

Article Title: Stromal Cells Positively and Negatively Modulate the Growth of Cancer Cells: Stimulation via the PGE2-TNFα-IL-6 Pathway and Inhibition via Secreted GAPDH-E-Cadherin Interaction

doi: 10.1371/journal.pone.0119415

Figure Lengend Snippet: ( A ) MKN-7 and MKN-74 cells were cultured for 15 min with or without 50 μg/mL anti-IL-6 neutralizing antibody in Hs738 CM prepared by 2 days of culture. The activation of STAT3 was analyzed by Western blotting. ( B ) MKN-7 and MKN-74 cells were cultured for 15 min with IL-6. The activation of STAT3 was analyzed by Western blotting. ( C ) MKN-7 and MKN-74 cells were cultured with IL-6 or CXCL1 for 3 days. Cell growth was determined by measuring GFP fluorescence intensity. The values are means ± s.d. (n = 3). ( D ) MKN-7 and MKN-74 cells were cultured alone (Mono) or co-cultured with Hs738 cells (Cocul) with the indicated antibodies for 3 days. Cell growth was determined by measuring GFP fluorescence intensity. The values are means ± s.d. (n = 3). Cell growth is expressed as a percentage of the value without antibodies in each culture condition.

Article Snippet: Anti-human IL-6 neutralizing antibody (MAB206), recombinant human IL-6 (206-IL), and recombinant human CXCL1 (275-CR/CF) were purchased from R&D Systems.

Techniques: Cell Culture, Activation Assay, Western Blot, Fluorescence

Journal: The Journal of Biological Chemistry

Article Title: Pyroglutamate and O -Linked Glycan Determine Functional Production of Anti-IL17A and Anti-IL22 Peptide-Antibody Bispecific Genetic Fusions

doi: 10.1074/jbc.M112.417717

Figure Lengend Snippet: The peptide-antibody genetic fusion neutralizes the IL22 activity equivalent to parental anti-IL22 antibody while lacking anti-IL17A neutralizing activity. A, kinetic analysis with human IL22 using a Biacore 2000. B, IL22-stimulated release of GRO-α production in HT29 cells. C, table summary of kinetic analysis with human IL17A using a Biacore 2000. D, IL17A-stimulated release of GRO-α in BJ human foreskin fibroblast cells. Error bars, S.D.

Article Snippet: Bound GRO-α was detected with anti-human GRO-α biotin (catalog no. BAF275, R&D Systems) at 40 ng/ml, followed by streptavidin-HRP (catalog no. DY998, R&D Systems), followed by a 1:1 mixture of H 2 O 2 and tetramethylbenzidine substrate (catalog no. DY999, R&D Systems), followed by 2 n H 2 SO 4 .

Techniques: Activity Assay

Journal: Frontiers in Cell and Developmental Biology

Article Title: Acetylcholine in the gingival epithelium drives the pathogenesis of periodontitis

doi: 10.3389/fcell.2025.1701252

Figure Lengend Snippet: Acetylcholine orchestrates a multi-dimensional defense program in epithelial cells. (A) Network of GSEA-GO enrichment analysis for Pg vs. NC DEGs. Each node represents a GO term, with circle size and color indicating the pathway size and the NES score, respectively. Node-sharing clusters represent functionally related term groups. (B) Transcript counts of CXCL and CCL family genes in HOKs RNA-seq data. Only genes with non-zero counts across all 12 samples are displayed, with orange bars indicating the mean. (C) Volcano plot of DEGs in Pg vs. NC comparison. Colored dots represent significantly differential expression (|log2 fold change| > 1, adjusted p -value <0.05). (D) Volcano plot of PD vs. HC DEGs in epithelial cells from single-cell data. Colored dots represent significantly differential expression (|log2 fold change| > 1, adjusted p -value <0.05). (E) Enzyme-linked immunosorbent assay (ELISA) results of HOK supernatants for CXCL1 and CXCL8. *, p < 0.05 and **, p < 0.01. (F) Enrichment analysis of DEGs from the Pg + Ach vs. Pg groups using the fgsea function, showing four significantly enriched functional categories [adjusted p < 0.05, normalized enrichment score (NES) > 1.5].

Article Snippet: The levels of CXCL1 and CXCL8 were then measured using an enzyme-linked immunosorbent assay (ELISA) kit (Proteintech, KE00133 and KE00453), according to the manufacturer’s instructions.

Techniques: RNA Sequencing, Comparison, Quantitative Proteomics, Enzyme-linked Immunosorbent Assay, Functional Assay

Journal: Oncology Letters

Article Title: CXCL1 expression is correlated with Snail expression and affects the prognosis of patients with gastric cancer

doi: 10.3892/ol.2015.3614

Figure Lengend Snippet: Expression of chemokine (C-X-C motif) ligand in gastric cancer and para-carcinoma tissues, as determined by immunohistochemistry. (A) CXCL1-positive and (B) CXCL1-negative tumor tissue; and (C) CXCL1-negative normal tissue (streptavidin-peroxidase method; magnification, x200).

Article Snippet: Mouse anti-human CXCL1 monoclonal antibody (dilution, 1:50; catalog no., MAB275; R&D Systems, Inc., Minneapolis, MN, USA) and rabbit anti-human Snail monoclonal antibody (dilution, 1:50; catalog no., ab180714; Abcam, Cambridge, UK) were used.

Techniques: Expressing, Immunohistochemistry

Journal: Oncology Letters

Article Title: CXCL1 expression is correlated with Snail expression and affects the prognosis of patients with gastric cancer

doi: 10.3892/ol.2015.3614

Figure Lengend Snippet: Univariate analysis of factors associated with the overall survival rate.

Article Snippet: Mouse anti-human CXCL1 monoclonal antibody (dilution, 1:50; catalog no., MAB275; R&D Systems, Inc., Minneapolis, MN, USA) and rabbit anti-human Snail monoclonal antibody (dilution, 1:50; catalog no., ab180714; Abcam, Cambridge, UK) were used.

Techniques: Expressing

Journal: Oncology Letters

Article Title: CXCL1 expression is correlated with Snail expression and affects the prognosis of patients with gastric cancer

doi: 10.3892/ol.2015.3614

Figure Lengend Snippet: Multivariate analysis of CXCL1 expression associated with the overall survival rate.

Article Snippet: Mouse anti-human CXCL1 monoclonal antibody (dilution, 1:50; catalog no., MAB275; R&D Systems, Inc., Minneapolis, MN, USA) and rabbit anti-human Snail monoclonal antibody (dilution, 1:50; catalog no., ab180714; Abcam, Cambridge, UK) were used.

Techniques: Expressing

Journal: Oncology Letters

Article Title: CXCL1 expression is correlated with Snail expression and affects the prognosis of patients with gastric cancer

doi: 10.3892/ol.2015.3614

Figure Lengend Snippet: Kaplan-Meier analysis of the overall survival time of patients with gastric cancer, based on CXCL1 and Snail expression. (A) High CXCL1 expression in gastric cancer tissue was associated with a poor overall survival time (P<0.001). (B) High Snail expression was also associated with a worse overall survival time (P<0.001). CXCL1, chemokine (C-X-C motif) ligand.

Article Snippet: Mouse anti-human CXCL1 monoclonal antibody (dilution, 1:50; catalog no., MAB275; R&D Systems, Inc., Minneapolis, MN, USA) and rabbit anti-human Snail monoclonal antibody (dilution, 1:50; catalog no., ab180714; Abcam, Cambridge, UK) were used.

Techniques: Expressing

Journal: Oncology Letters

Article Title: CXCL1 expression is correlated with Snail expression and affects the prognosis of patients with gastric cancer

doi: 10.3892/ol.2015.3614

Figure Lengend Snippet: Association between CXCL1 and Snail expression levels and clinical characteristics of patients with gastric cancer.

Article Snippet: Mouse anti-human CXCL1 monoclonal antibody (dilution, 1:50; catalog no., MAB275; R&D Systems, Inc., Minneapolis, MN, USA) and rabbit anti-human Snail monoclonal antibody (dilution, 1:50; catalog no., ab180714; Abcam, Cambridge, UK) were used.

Techniques: Expressing

Journal: Oncology Letters

Article Title: CXCL1 expression is correlated with Snail expression and affects the prognosis of patients with gastric cancer

doi: 10.3892/ol.2015.3614

Figure Lengend Snippet: Association between Snail expression and CXCL1 expression, determined using Fisher's exact test. CXCL1, chemokine (C-X-C motif) ligand.

Article Snippet: Mouse anti-human CXCL1 monoclonal antibody (dilution, 1:50; catalog no., MAB275; R&D Systems, Inc., Minneapolis, MN, USA) and rabbit anti-human Snail monoclonal antibody (dilution, 1:50; catalog no., ab180714; Abcam, Cambridge, UK) were used.

Techniques: Expressing

Journal: Oncology Letters

Article Title: CXCL1 expression is correlated with Snail expression and affects the prognosis of patients with gastric cancer

doi: 10.3892/ol.2015.3614

Figure Lengend Snippet: Multivariate analysis of CXCL1 and Snail expression associated with the overall survival rate.

Article Snippet: Mouse anti-human CXCL1 monoclonal antibody (dilution, 1:50; catalog no., MAB275; R&D Systems, Inc., Minneapolis, MN, USA) and rabbit anti-human Snail monoclonal antibody (dilution, 1:50; catalog no., ab180714; Abcam, Cambridge, UK) were used.

Techniques: Expressing